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KMID : 0358419960390010176
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 1 p.176 ~ p.185
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Seroreactivites to HPV-16 Virus Like Particles(VLPs) using ELISA in the Patients with Cervical Neoplasia
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박종섭
황은성/안희경/김찬주/정근택/김현수/박순희
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Abstract
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Backgroud:
The late structural proteins of HPV-16 have recently been shown to self-assemble into virus-like particles(VLPs) from baculovirus system when expressed in insect cells.
The ability to generate preparative amounts of HPV-16 L1-L2 VLP may have implications for the development of a serologic assay to detect anti-HPV-16 virion immune responses to conformational epitopes and for immunoprophylaxis against HPV-16
infection.
Methods:
We attempted to investigate serologic responses in the sera obtained from Korean women with cervical neoplasia by ELISA using HPV-16 VLPs. PCR method using LI consensus primers and type-specific oligomer probes was used to determine the
presence and
type of HPV infection in the study population(normal controls ; 23 cases, preinvasive lesions ; 24 cases, and invasive cervical cancers ; 96 cases).
Results:
1) The sera of 93%(21/23) of women, who didn't have HPV-related cervical lesions, were negative for HPV-16 VLPs in ELISA assay. The sera of 50%(12/23) of women, who didn't have HPV-related cervical lesions, were negative for HPV-16 VLPs in
ELISA
assay. The sera of 50%(12/24) of patients with preinvasive cervical lesion and 63%(60/96) of patients with invasive cervical cancer were ELISA positive for HPV-16 VLPs(p<0.01). 2) The positive reactivities for ELISA of HPV-16 VLPs in cervical
cancer
patients according to clinical characteristics were not signific-antly different. 3) Among women with cervical neoplasia, HPV-16 VLP was positive in 50%(10/20) of HPV-16 positive cases with preinvasive cervical lesion and 72%(50/72) of HPV-16
positive
cases with invasive cervical cancer.
Conclusion:
These results suggest that the considerable number of patients with cervical neoplasia generated an IgG antibody response to the HPV-16 VLPs that can be detected by ELISA. These HPV-16VLP ELISA may be useful in adjunctive diagnostic assay to
HPV-16
DNA test and seroepidemiologic study of HPV-related cervical neoplasia.
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KEYWORD
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